Efficacy of System

System Efficacy - Microbial De-Contamination

NABL Certified Agency appointed to test and validate the efficacy of the system.
Testing facility - 2000 Square Ft. Sterilized room with ducted supply and return air.
Temperature was maintained @ 23 deg C and 50% Rh to simulate a commercial building environment.
2 Test plates, one coated with Nano TiO₂ and another without were infected with a consortium of Micro-Organisms and place inside the AHU.

Sr.	Micro-Organisms	Category	Culture Media
1	Staphylococcus aureus	Gram Positive Bacteria	Vojel Johnson (V J) agar
2	Escherichia coli	Gram Negative Bacteria	Eosin Methylene Blue (EMB) agar
3	Pseudomonas aeruginosa	Gram Negative Bacteria	Cetrimide agar
4	Candida albicans	Yeast	Sabourauds Dextrose agar with Antibiotic
5	Aspergillus niger	Mold	Sabourauds Dextrose agar with Antibiotic

Swab samples from treated and untreated plates are collected and spread over different Culture media (agar plates) to capture specific micro-organism count.
To monitor the reduction in CFU in air, samples are collected with an Anderson sampler and Gravimetric method.
The samples are collected at interval of 0 hour to get initial count and further samples are collected after 15 minutes and 180 minutes.

0 min
100,000 TCFU

15 min
231 TCFU

180 min
15 TCFU

0 min
100,000 TCFU

15 min
231 CFU

180 min
15 CFU

15 min
45 TCFU

180 min
0 TCFU

15 min
45 TCFU

180 min
0 CFU

IGBC parameter for Indoor Emission & Microbial Count	Threshold value Class A	Threshold value Class B
Total Microbial Count	50 CFU/m³	150 CFU/m³

A mixture of Paint thinner, Cleaning Chemicals, Sanitary Deodorizer & Aerosols (Room freshener) were introduced into the air conditioned space, simulating TVOC counts above Toxic Limits.
The Graywolf Sensors were setup for monitoring.
Upon stabilization of TVOC reading, the data logger is started to record the TVOC count and monitor TVOC reduction with the IAQURE system in real time.
Logging was stopped when the VOC & Formaldehyde count reached the permissible count of 500 µgm/m³
The process was repeated for the same timeframe without the PCO to determine the TVOC reduction and determine the efficacy of the system.

Benchmark with IGBC – Healthy Building Standards for Micro-organism and TVOC control
Customer	Microbial Count (CFU) Sample Surface Area 25 Sq. cm²			Microbial Count (CFU/m³) Air Sampling		TVOC (µg/m³)
Customer	Pre Installation Oct 2017	Post Installation Dec 2017	Post Installation Mar 2018	Post Installation Mar 2018	IGBC Class	Post Installation Dec 2017	Post Installation Mar 2018	IGBC Class<
Customer 1	1000++	0	8	77	B	60	122	A
Customer 2	1000++	1	20	48	A	357	157	A
Customer 3	1000++	56	4	34	A	75	125	A
Customer 4	1000++	55	4	28	A	89	147	A
Customer 5	1000++	2	10	127	B	201	140	A
IGBC Threshold values for Microbial Count & VOC & Formaldehyde : 50 CFU/m³ & 500 µg/m³ for “ Class A “ 150 CFU/m³ & 650 µg/m³ for “ Class B” respectively , as per ASHRAE standard .